Статья 'Создание автоматизированной системы анализа изображений полимеразной цепной реакции' - журнал 'Кибернетика и программирование' - NotaBene.ru
по
Journal Menu
> Issues > Rubrics > About journal > Authors > About the Journal > Requirements for publication > Council of Editors > Peer-review process > Policy of publication. Aims & Scope. > Article retraction > Ethics > Online First Pre-Publication > Copyright & Licensing Policy > Digital archiving policy > Open Access Policy > Open access publishing costs > Article Identification Policy > Plagiarism check policy
Journals in science databases
About the Journal

MAIN PAGE > Back to contents
Cybernetics and programming
Reference:

Building of an automated system for the images of polymerase chain reaction analysis

Ipatov Yurii Arkad'evich

PhD in Technical Science

Associate Professor of the Department of Informatics at the Volga State University of Technology

424000, Russia, Mari El, Yoshkar-Ola, pl. Lenina, d. 3

philsilver@mail.ru
Другие публикации этого автора
 

 
Novikov Petr Sergeevich

Assistant of the Department of Forest Breeding, Biotechnology and Non-timber Resources at the Volga State University of Technology

424000, Russia, Mari El, Yoshkar-Ola, pl. Lenina, d. 3

philsilver@mail.ru
Shurgin Aleksei Ivanovich

Associate Professor of the Department of Forest Breeding, Biotechnology and Non-timber Resources at the Volga State University of Technology

424000, Russia, Mari El, Yoshkar-Ola, pl. Lenina, d. 3

philsilver@mail.ru

DOI:

10.7256/2306-4196.2013.6.10323

Review date:

17-11-2013


Publish date:

1-12-2013


Abstract: The article discusses the question of automated processing and analysis of the gels images gathered as a result of electrophoresis of samples containing products of polymerase chain reaction (PCR). The proposed approach allows to significantly increase the speed of the DNA-analysis. The authors state that one of the  resource and time-consuming stages of the forming of initial data is the analysis of digital images of the results of chemical reaction. The article reviews the development of necessary theoretical methods and practical implementations of automated image analysis. It is noted that one of the  resource and time-consuming stages of the forming of initial data is the analysis of digital images of the results of chemical reaction. The presented development of an automated system based on the methods of pattern recognition and analysis of digital images of gels allows to minimize the time-consuming operation performed manually and to increase the quality of the result. The article brings the algorithm for automated recognition and assigning of unique lines that define the field of primers and products of PCR. Comparative analysis of the manual methods and created approach shows that the automated system is one and a half order of values faster. The massive usage and accuracy of this method also increases the economic benefits from the use of this product.


Keywords: computer-aided system, image analysis, polymerase chain reaction, DNA analysis, primer, image gels, electrophoresis, signal, filtering, software developers
This article written in Russian. You can find full text of article in Russian here .

References
1.
SaikiR.K., GelfandD.H., StoffelS., HiguchiR. // Science. 1988. V.239. N.2. Р.487.
2.
П.С. Новиков, О.В. Шейкина, Т.Н. Милютина Изменчивость плюсовых деревьев сосны обыкновенной на архиве клонов по ISSR-маркерам // Вестник МарГТУ. Радиотехнические и инфокоммуникационные системы. – Йошкар-Ола: МарГТУ– №.3, 2011. – С.82-87.
3.
Самарский, А. А.Численные методы / А. А. Самарский, А. В. Гулин – М.:Наука, 1989. –432 с.
4.
Тихонов, В. И. Оптимальный прием сигналов/ В.И. Тихонов – М.: Радио и связь, 1983. – 320 с.
5.
Прэтт У. Цифровая обработка изображений: в 2 кн. М.: Мир, 1982.– 790 с.
6.
Ипатов Ю.А., Кревецкий А.В., Шмакин В.О. Проектирование распределенной наземной системы мониторинга за лесными пожарами // NB: Кибернетика и программирование. - 2013. - 2. - C. 20 - 28. URL: http://www.e-notabene.ru/kp/article_8309.html
7.
Ю.А. Ипатов, А.В. Кревецкий Обнаружение текстурных переходов на изображениях препаратов клеточной структуры древесины // Программные системы и вычислительные методы. - 2013. - 1. - C. 106 - 115. DOI: 10.7256/2305-6061.2013.01.9.
8.
А.Г. Коробейников, С.С. Кувшинов, С.Ю. Блинов, А.В. Лейман Анализ принципов создания и работы стеганографических алгоритмов // Программные системы и вычислительные методы. - 2012. - 1. - C. 28 - 36.
Link to this article

You can simply select and copy link from below text field.


Other our sites:
Official Website of NOTA BENE / Aurora Group s.r.o.
"History Illustrated" Website