Abstract: The subject of this research is the work of university museum “Archeology, Ethnography, and Ecology of Siberia” pertaining to actualization of the objects of historical-cultural and natural heritage in form of ethnographical and entomological collections of the museum, which in turn are viewed as the object of this research. The goal of the article consists in determination of the peculiarities of actualization of heritage within the framework of cultural-educational activity of the museum, as well as substantiation of the approaches, methods, and recommendations that contribute into optimization of the activity, when the collections based on museum methods are included into the modern sociocultural space. Analysis of actualization of museum collection as a whole, considering the specific characteristics of ethnocultural and natural heritage, is based on the systemic methodological approach. Scientific novelty of this article manifests in the necessity of identification of opportunities to include the various types of heritage into the modern museum space, as well as improve the theoretical base on actualization of such heritage with regards to the museum practices. The research reveal the extensive opportunities and prospects of actualization not just the ethnographical materials, but also entomological collections.