Abstract: The subject of this research is the main characteristics of the preschool polycultural subject-spatial environment. The author carefully examines such aspects as the characteristics of the preschool polycultural subject-spatial environment based on the scales for a complex assessment of the quality of education in preschool organizations ECERS. According to the parameter “Organization of play space” the following features are being revealed: “At least five different centers of interest allow the children to acquire various learning experience”; “The centers are organized so the children can use them independently”; “The additional materials are available for the purpose of making changes in the structure of the centers”. Special attention is given to the centers of interests of the children in subject-spatial environment. During the course of this research the author used the following methodology: The ideas of connection, interconditionality, and integration of the phenomena and processes of the surrounding world, wholeness of an individual, its natural, social, and cultural essence (B. A. Ananyev, I. S. Kon, and others); The ideas of upbringing as a process of individual development and formation of values of the preschooler (L. V. Abdulmanova, I. E. Kulikovskaya, N. E. Tatarintseva, R. M. Chumicheva, and others); The concept of polycultural education (O. V. Gukalenko, G. S. Denisova. L. L. Suprunova, and others); The theory of polycultural educational space (V. P. Borisenkov, O. V. Gukalenko, A. Y. Danilyuk). The author concludes that the polycultural subject-spatial environment is in high demand if the main educational value consists in the development of child’s personality, as well as his self-realization. Such environment creates a field for the cognitive development of a child, emergence of his own understandable world that reflects the living space. The scientific novelty lies in examination of the preschool polycultural subject-spatial environment based in accordance with the international requirements.